About the Author Preface Guide to Experimental Techniques in Molecular Biology Brief Contents Brief Table of Contents PART Ⅰ Introduction 1 A Brief History 2 The Molecular Nature of Genes 3 An Introduction to Gene Function PART Ⅱ Methods in Molecular Biology 4 Molecular Cloning Methods 5 Molecular Tools for Studying Genes and Gene Activity PART Ⅲ Transcription in Prokaryotes 6 The Transcription Apparatus of Prokaryotes 7 Operons:Fine Control of Prokaryotic Transcription 8 Major Shifts in Prokaryotic Transcription 9 DNA-Protein Interactions in Prokaryotes PART Ⅳ Transcription in Eukaryotes 10 Eukaryotic RNA Polymerases and Their Promoters 11 General Transcription Factors in Eukaryotes 12 Transcription Activators in Eukaryotes 13 Chromatin Structure and Its Effects on Transcription PART Ⅴ Post-Transcriptional Events 14 Post-Transcriptional Events Ⅰ:Splicing 15 Post-Transcriptional Events Ⅱ:Capping and Polyadenylation 16 Post-Transcriptional Events Ⅲ:Other Events PART Ⅵ Translation 17 The Mechanism of Translation Ⅰ:Initiation 18 The Mechanism of Translation Ⅱ:Elongation and Termination 19 Ribosomes and Transfer RNA PART Ⅶ DNA Replication,Recombination,and Transposition 20 DNA Replication Ⅰ:Basic Mechanism and Enzymology 21 DNA Replication Ⅱ:Detailed Mechanism 22 Recombination and Transposition Glossary Credits Index