目录 序 前言 上 第1部分 生物固氮研究 我国生物固氮研究的现状和对策 4 花生根瘤菌自生固氮的研究 8 耐盐高效大豆根瘤菌株的构建 15 苜蓿根瘤菌 (Rhizobium meliloti) 的耐盐性研究 21 红豆草根瘤菌的耐盐和共生性状改造 28 Tn5-Mob系统诱导根瘤菌属之间耐盐和共生性状的转移 33 北京郊区固氮螺菌的研究 38 酶联免疫吸附技术(ELISA)对大豆根瘤菌的鉴定 46 肺炎克氏杆菌的nifA基因产物在巴西固氮螺菌中的功效 52 巴西固氮螺菌(Azospirillum brasilense)Yu62的质粒及nif HDK基因的定位 61 肺炎克氏杆菌nifA基因在巴西固氮螺菌固氮基因表达的铵调节中的作用 67 几种固氮菌nifA基因片段的同源性分析 71 巴西固氮螺菌ntrBC基因的克隆与核苷酸序列分析 76 肺炎克氏杆菌NifA对巴西固氮螺菌nifH启动子的转录激活作用 83 巴西固氮螺菌Yu62 draTG基因启动子区域的核苷酸序列及其功能分析 89 巴西固氮螺菌Yu62 draTG基因及其下游区域的定位诱变分析 97 巴西固氮螺菌Yu62 nifA基因克隆、测序及功能分析 103 巴西固氮螺菌鞭毛调节基因flbD鉴定 109 巴西固氮螺菌Yu62 glnB基因的克隆及其功能分析 115 玉米联合固氮工程菌的增产和节约氮肥的效果 122 Interaction Between PII and NifA in Azospirillum brasilense Sp7 126 Functional Analysis of the GAF Domain of NifA in Azospirillum brasilense: Effects of Tyr → Phe Mutations on NifA and Its Interaction with GlnB 133 PAS Domain of the Deduced Org35 Protein Mediates the Interaction with NifA 144 Isolation of a Flagellar Operon in Azospirillum brasilense and Functional Analysis of FlbD 149 Interaction Between GlnB and the N-terminal Domain of NifA in Azospirillum brasilense 161 A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli 171 Comparative Genomic Analysis of N2-Fixing and Non-N2-Fixing Paenibacillus spp.: Organization, Evolution and Expression of the Nitrogen Fixation Genes 188 Positive and Negative Regulation of Transferred nif Genes Mediated by Indigenous GlnR in Gram-positive Paenibacillus polymyxa 212 Combined Assembly and Targeted Integration of Multigene for Nitrogenase Biosynthetic Pathway in Saccharomyces cerevisiae 234 Influence of pN2 and pD2 on HD Formation by Various Nitrogenases 248 固氮酶催化的放H2反应 265 肺炎克氏杆菌固氮酶双突变株的构建及其对底物还原的特性 275 N2 和H+在固氮酶活性中心金属原子簇中还原位点的分析 285 Mutagenesis at α-423Ile of MoFe Protein Reduces the Catalytic Activity of Nitrogenase in Klebsiella oxytoca 294 α-Lys424 Participates in Insertion of FeMo-co to MoFe Protein and Maintains Nitrogenase Activity in Klebsiella oxytoca M5al 305 第2部分微生物次生代谢产物研发及生物合成调控研究 北京农业大学的赤霉素研究工作(简报) 326 赤霉素A4、A7的发酵研究 330 赤霉素A9检定方法的建立和发酵的初步研究 337 莫能菌素应用技术 343 透明颤菌血红蛋白基因在阿维链霉菌中的表达 345 阿维菌素高产菌株的选育及阿维菌素B1的鉴定 352 Avermectins发酵培养基的研究 358 阿维链霉菌中aveD基因缺失对阿维菌素合成的影响 366 Deletion Analysis of Oligomycin PKS Genes (olmA) in Streptomyces avermitilis 371 Construction of Ivermectin Producer by Domain Swaps of Avermectin Polyketide Synthase in Streptomyces avermitilis 380 An adpA Homologue in Streptomyces avermitilis is Involved in Regulation of Morphogenesis and Melanogenesis 392 Engineering of Avermectin Biosynthetic Genes to Improve Production of Ivermectin in Streptomyces avermitilis 403 Enhancement of Avermectin and Ivermectin Production by Overexpression of the Maltose ATP-binding Cassette Transporter in Streptomyces avermitilis 410 A New Strain of Streptomyces avermitilis Produces High Yield of Oligomycin A with Potent Anti-tumor Activity on Human Cancer Cell Lines in vitro 423 Chromosomal Instability in Streptomyces avermitilis: Major Deletion in the Central Region and Stable Circularized Chromosome 433 The Pathway-specific Regulator AveR from Streptomyces avermitilis Positively Regulates Avermectin Production while it Negatively Affects Oligomycin Biosynthesis 450 Increasing Avermectin Production in Streptomyces avermitilis by Manipulating the Expression of a Novel TetR-family Regulator and Its Target Gene Product 466 AvaR2, a Pseudo γ-Butyrolactone Receptor Homologue from Streptomyces avermitilis, is a Pleiotropic Repressor of Avermectin and Avenolide Biosynthesis and Cell Growth 490 SAV742, a Novel AraC-Family Regulator from Streptomyces avermitilis, Controls Avermectin Biosynthesis, Cell Growth and Development 513 AvaR1, a Butenolide-type Autoregulator Receptor in Streptomyces avermitilis, Directly Represses Avenolide and Avermectin Biosynthesis and Multiple Physiological Responses 532 Redox-sensing Regulator Rex Regulates Aerobic Metabolism, Morphological Differentiation, and Avermectin Production in Streptomyces avermitilis 552 The PhoP Transcription Factor Negatively Regulates Avermectin Biosynthesis in Streptomyces avermitilis 568 IdeR, a DtxR-family Iron-response Regulator, Controls Iron Homeostasis, Morphological Differentiation, Secondary Metabolism, and Oxidative Stress Response in Streptomyces avermitilis 583 AveI, an AtrA Homolog of Streptomyces avermitilis, Controls Avermectin and Oligomycin Production, Melanogenesis, and Morphological Differentiation 604 SufR, a [4Fe-4S] Cluster-containing Transcription Factor, Represses the sufRBDCSU Operon in Streptomyces avermitilis Iron-sulfur Cluster Assembly 621 The ROK-family Regulator Rok7B7 Directly Controls Carbon Catabolite Repression, Antibiotic Biosynthesis, and Morphological Development in Streptomyces avermitilis 642 AccR, a TetR Family Transcriptional Repressor, Coordinates Short-chain Acyl Coenzyme A Homeostasis in Streptomyces avermitilis 666 BldD, a Master Developmental Repressor, Activates Antibiotic Production in two Streptomyces Species 683 自养黄杆菌合成羟基丁酸和羟基戊酸共聚体的发酵研究 710 真养产碱杆菌聚羟基烷酸合成酶基因在欧文氏菌中的表达 718 高产稳产聚羟基烷酸的重组大肠杆菌的构建 724 Fermentation of 1, 3-Propanediol by a Lactate Deficient Mutant of Klebsiella oxytoca Under Microaerobic Conditions 730 Influence of Blocking of 2, 3-Butanediol Pathway on Glycerol Metabolism for 1, 3-Propanediol Production by Klebsiella oxytoca 741
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