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Biochemistry and molecular biology laboratory course(生物化学与分子生物学实验教程 英文版)


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Biochemistry and molecular biology laboratory course(生物化学与分子生物学实验教程 英文版)
  • 书号:9787030418968
    作者:徐跃飞,田余祥,孔英
  • 外文书名:
  • 装帧:平装
    开本:16
  • 页数:103
    字数:151000
    语种:zh-Hans
  • 出版社:科学出版社
    出版时间:2014-09-01
  • 所属分类:Q58 生物体其他化学成分
  • 定价: ¥48.00元
    售价: ¥38.40元
  • 图书介质:
    纸质书 电子书

  • 购买数量: 件  可供
  • 商品总价:

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本书针对生物化学教学及研究中涉及的三类主要的生物分子——蛋白质、酶和核酸,根据生物大分子的理伦性质,结合实用的电泳技术、层次技术、离心技术、分子克隆技术等,综合系统地介绍生物大分子的分离、纯化、含量测定、分析鉴定和应用,并结合教学中物质代谢、酶促反应动力学的系统分析,设计实验并融入相关实验技术和研究新进展,使学生在实验教学中掌握知识、技能的同时,体会对科研方法的选择与评价。
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目录

  • Contents
    Part 1 Basic techniques of biochemistry and molecular biology
    Chapter 1 Basic techniques of preparation of biomacromolecules l
    Section 1 Salting out 2
    Section 2 Dialysis and ultrafiltration 3
    Chapter 2 Spectrophotometry 5
    Section 1 Basic principles 5
    Section 2 The basic construction of spectrophotometers 7
    Section 3 Some home-made spectrophotometers 9
    Chapter 3 Chromatography 12
    Section 1 Adsorption chromatography 12
    Section 2 Partition chromatography 12
    Section 3 Ion-exchange chromatography 14
    Section 4 Gel filtration 16
    Section 5 Affinity chromatography 18
    Chapter 4 Electrophoretic techniques 20
    Section 1 Basic principles 20
    Section 2 Several types of commonly used electrophoresis 23
    Chapter 5 Centrifugation techniques 29
    Section 1 Basic principle 29
    Section 2 General description of centrifugation 30
    Chapter 6 Genetic engineering and analytical technology of gene expression 34
    Section 1 Genetic engineering 34
    Section 2 Polymerase chain reaction 40
    Section 3 Nucleic acid hybridization 45
    Section 4 DNA sequencing technology 46
    Part 2 Experiments of the biochemistry and molecular biology
    Chapter 7 Analysis of purification and physicochemical properties of proteins 49
    Experiment 1 Colorimetric methods for protein determination 49
    Experiment 2 Isolation and purification of serum y-globulin 51
    Experiment 3 Cellulose acetate membrane electrophoresis of serum proteins 53
    Experiment 4 Determination of relative molecular mass of protein in SDS-PAGE 55
    Experiment 1 The specificity of enzyme and effect of temperature, pH on the enzyme Activity 58
    Experiment 2 Isolation of alkaline phosphatase and determination of its specific activity 60
    Experiment 3 Effects of substrates concentration of enzymatic activity: determination of Xm of alkaline phosphatase 64
    Experiment 4 Inhibition effect of phosphate on alkaline phosphatase 66
    Experiment 5 Analysis of lactic dehydrogenase isoenzyme 67
    Chapter 9 Substance metabolism 70
    Experiment 1 Extraction and identification of liver glycogen 70
    Experiment 2 Determination of alanine transaminase activity (Reitman-Frankels’method) 71
    Experiment 3 Enzyme catalyzed transamination of amino acids detected by paper chromatography 73
    Chapter 10 Basic experiment of molecular biology 76
    Experiment 1 Preparation of the genomic DNA in the eukaryotic cells 76
    Experiment 2 Determination of the concentration and purity of DNA by spectroscopy 77
    Experiment 3 Agarose gel electrophoresis of nucleic acids 77
    Experiment 4 Polymerase chain reaction (PCR) 79
    Experiment 5 Isolation and purification of the DNA fragments 80
    Experiment 6 DNA ligation^constructing recombinant plasmids 82
    Experiment 7 Preparation of E.coli competent cells 83
    Experiment 8 Transformation of Ca2+ competent cells 84
    Experiment 9 The screening of recombinant DNA 85
    Experiment 10 Minipreparation of plasmid DNA 86
    Experiment 11 Digestion of plasmid DNA with restriction endonucleases 89
    Experiment 12 Southern blot 90
    Experiment 13 Expression of exogenous gene in prokaryotic cells 93
    References 95
    Appendix 96
    Appendix Ⅰ The calculation table of ammonium sulfate saturation 96
    Appendix Ⅱ Common buffer solution preparation 96
    Appendix Ⅲ The relative molecular mass and pi of protein 100
    Appendix Ⅳ Common data of chromatography 101
    Appendix Ⅴ The conversion between centrifugation speed of centrifugation force 103
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