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  生物技术概论（双...
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本书为国家双语教学示范课程配套教材，全书分为基因克隆、细胞工程、酶技术、工业发酵四个部分，共21章，分别介绍了上述四大生物技术领域的基础知识、技术关键以及发展前沿与动态，并对有关技术的正负面影响提出思辨。附录部分提供了关键词汇的中英文对照;列出了本领域诺贝尔奖获得者名单及其主要贡献;提供了近十年来出版的英文原版专业书目，以利于读者自学、参考。

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• Contents
  Preface
  Part I Gene Cloning
  Chapter 1 Introduction to Gene Cloning 1
  R.R Brief Overview R
  R.2 Historical Events 3
  R.3 Significance and Alertness 5
  Chapter 2 Gene and Genome 7
  2.R Discovery of Hereditary Factor 7
  2.2 Basic Knowledge of Gene 14
  2.3 Sequence Signals Involved with Gene Expression 22
  2.4 About Genomic and Metagenomic Studies 26
  Chapter 3 Basic Techniques for Experiments with DNA 28
  3.R Enzymatic Tools for Gene Manipulation 28
  3.2 Oligo-nucleotide Tools 34
  3.3 DNA Purification 36
  3.4 Electrophoresis 38
  3.5 In Vitro Synthesis of DNA 40
  3.6 DNA Sequencing 43
  3.7 Macromolecular Hybridization and Blotting 46
  3.8 PCR Techniques 48
  Chapter 4 Cloning Procedure 53
  4.R Basic Procedure of Cloning 53
  4.2 Selection of Cloning Vectors 54
  4.3 Preparation of Donor DNA 60
  4.4 Joining of Donor and Vector by Ligation 62
  4.5 Selectio n of H o st Cells 62
  4.6 Introduction of Recombinant DNA into Host Cells 64
  4.7 Selection，Screening and Sub-cloning.65
  4.8 Derivative Technology Based on CloNng 69
  Chapter 5 Application and Impacts 73
  5.1 Initiation of Recombinant DNA Industry 73
  5.2 General Aspects of Application 77
  5.3 Contribution and Impact 83
  Part II Cell Engineering
  Chapter 6 Introduction on Cell Engineering 88
  6.1 Brief Overview 88
  6.2 Essentials of Cell Engineering 89
  6.3 Progress Clues 91
  Chapter 7 Cell Fusion and Hybridization 94
  7.1 General Aspects 94
  7.2 Protoplast Technique 94
  7.3 Basic Methods of Cell Fusion 97
  7.4 Fusant Screening 98
  7.5 Application of Cell Fusion 99
  Chapter 8 Plant Tissue and Cell Culture 100
  8.1 Plant Tissue Culture System 100
  8.2 Gene Transfer to Plants 108
  8.3 Artificial Seeds and Virus-free Plants 113
  Chapter 9 Animal Tissue and Cell Culture 116
  9.1 Establishment of Animal Tissue Culture System 116
  9.2 Gene Transfer to Animal Cell 120
  9.3 Animal Hybrids and Hybridoma 124
  9.4 Approaches of Stem Cell Research 129
  Chapter 10 Application Examples from Cell Engineering 132
  10.1 Bioproducts from Cell Engineering 132
  10.2 Breeding of New Agronomic Varieties 134
  10.3 Transgenic Animals as Models for Medical Research 138
  Part III Enzyme Technology
  Chapter 11 Fundamental Nature of Enzyme 141
  11.1 Definition, Basic Nature and Classification of Enzyme 141
  11.2 Determination of Enzyme Activities 1 46
  11.3 Factors Affecting Enzyme Activity 150
  11.4 Resources of Enzyme 1 57
  11.5 Uses of Enzyme 159
  Chapter 12 Enzyme Biosynthesis and Products 163
  12.1 Bio-resources of Enzyme 1 63
  12.2 About Enzyme Synthesis 170
  12.3 Enzyme Fermentation and Products R73
  Chapter 13 Enzyme Recovery&Purification 181
  13.R Pretreatment of Crude Enzyme Sample 181
  13.2 Removing of Impurities and Sample Concentration 182
  13.3 Chromatographic Separation 184
  13.4 Polishing of Enzyme 191
  13.5 Rational Formulation of Final Enzyme Products 192
  Chapter 14 Enzymatic Conversion 194
  14.1 Enzymatic Conversion 194
  14.2 Immobilization of Enzyme and Cells 200
  14.3 Enzymatic conversion in non-aqueous Phase 207
  Chapter 15 New Approaches of Enzyme Technology 211
  15.1 Modification on Enzymes 211
  15.2 Enzyme Carriers for Immobilization 216
  15.3 Enzyme Inhibitors 219
  15.4 Ribozyme (Non-protein Enzymes) 222
  Part IV Industrial Fermentation
  Chapter 16 Principles of Fermentation 225
  16.1 Fermentation Principles 225
  16.2 Fermentation Products and Application 228
  16.3 Benefits from the Fermentation Industry 231
  Chapter 17 Industrial Microorganisms 235
  17.1 Types and Characters of Industrial Microorganisms 235
  17.2 Breeding of Industrial Strains 240
  17.3 Industrial Microbial Growth and Culture Media 242
  17.4 Inoculation of Industrial Strains 249
  17.5 Preservation of Industrial Strains 252
  Chapter 18 Microbial Metabolism and Fermentation Kinetics 254
  18.1 Metabolism Related to Basic Pathway 254
  18.2 Metabolic Regulation and Control 262
  18.3 Stoichiometry of Fermentation Process 268
  18.4 Fermentation Kinetics 272
  Chapter 19 Bioreactors and Auxiliary Equipments 280
  19.1 Structure and Function of Bioreactors 280
  19.2 Auxiliary Equipment 285
  19.3 Probes and Sensors 288
  Chapter 20 Fermentation Down-Stream Technology 292
  20.1 General Aspects 292
  20.2 Harvest and Pretreatment 295
  20.3 Isolation and Purification 301
  20.4 Product Polishing 304
  20.5 Waste Treatment and Cleaner Production 306
  Chapter 21 Progress in Fermentation Engineering 311
  21.1 Approaches of Up-stream Technology 311
  21.2 Process Optima.318
  21.3 Development of Down-streamTechnology 321
  Appendix 325
  Terms Translation 325
  Related Nobel Laureates 336
  Further Reading 340
  后记 345