PCR技术是分子生物学研究和临床分子诊断的一种基本技术手段，数字PCR技术则是PCR技术和现代微机电技术相结合的产物。以PCR的理论和技术体系为基础，结合现代微机电和光学检测技术，实现单分子水平的核酸精确定量检测。数字PCR技术将DNA或RNA样品划分成单分子水平的微反应单元，然后对众多微反应单元内的靶序列进行扩增和光学检测，实现绝对定量，该技术的诞生和发展为核酸精确测定提供了一种全新的思路，并具有比传统实时荧光定量PCR技术更好的精确性和灵敏度。目前，该技术已经在肿瘤个体化诊疗、基因编辑、液体活检等生命科学研究及生物医学检测等多个领域实现应用。本书着重介绍了数字PCR技术特征、仪器系统发展、实验设计方法、试验数据处理及应用发展现状，并拓展介绍了一些实验操作和工程知识。
样章试读
目录
- 第1 章 数字聚合酶链反应技术概论 ································································· 1
第一节 引言 ························································································· 1
第二节 数字PCR 技术的发展历史 ····························································· 2
第三节 数字PCR 数学原理 ······································································ 6
第2 章 数字PCR 系统与发展 ········································································· 9
第一节 商业化平台 ················································································ 9
第二节 数字PCR 芯片与试剂关键技术 ······················································54
第三节 数字PCR 系统新发展 ··································································57
第3 章 数字PCR 实验设计 ···········································································74
第一节 概述 ························································································74
第二节 数字PCR 实验设计分类介绍 ·························································83
第三节 数字PCR 常见问题及解决方法 ······················································99
第4 章 数字PCR 的数学分析和定量特性研究 ··················································105
第一节 数字聚合酶链反应定量原理 ·························································105
第二节 数字PCR 统计学模型研究 ···························································111
第三节 数字PCR 的定量特性研究 ···························································123
第5 章 数字PCR 的应用 ·············································································134
第一节 数字PCR 应用特点 ···································································134
第二节 数字PCR 在基因组学研究中的应用 ···············································136
第三节 数字PCR 技术在转化医学和精准医疗中的应用 ································146
第四节 数字PCR 技术在食品安全检测中的应用 ·········································161
第五节 数字PCR 技术在环境微生物检测中的应用 ······································168
第六节 数字PCR 技术的其他应用 ···························································170
第七节 数字PCR 技术应用发展趋势 ························································176
第6 章 数字PCR 与其他基因分析技术的对比及其未来 ······································181
第一节 数字PCR 与其他基因分析技术的对比和融合 ···································181
第二节 数字PCR 技术的发展与未来 ························································186
敬启：·······································································································190
京公网安备 11010102004214号