• 
  Biochemi...
  ￥37.92元
• 
  生物化学（案例版...
  ￥63.04元

本书针对生物化学教学及研究中涉及的三类主要的生物分子——蛋白质、酶和核酸，根据生物大分子的理伦性质，结合实用的电泳技术、层次技术、离心技术、分子克隆技术等，综合系统地介绍生物大分子的分离、纯化、含量测定、分析鉴定和应用，并结合教学中物质代谢、酶促反应动力学的系统分析，设计实验并融入相关实验技术和研究新进展，使学生在实验教学中掌握知识、技能的同时，体会对科研方法的选择与评价。

样章试读

• 暂时还没有任何用户评论

总计 0 个记录，共 1 页。 第一页 上一页 下一页 最末页

全部咨询(共0条问答)

• 暂时还没有任何用户咨询内容

总计 0 个记录，共 1 页。 第一页 上一页 下一页 最末页

用户名：	匿名用户
E-mail：
咨询内容：

• Contents
  Part 1 Basic techniques of biochemistry and molecular biology
  Chapter 1 Basic techniques of preparation of biomacromolecules l
  Section 1 Salting out 2
  Section 2 Dialysis and ultrafiltration 3
  Chapter 2 Spectrophotometry 5
  Section 1 Basic principles 5
  Section 2 The basic construction of spectrophotometers 7
  Section 3 Some home-made spectrophotometers 9
  Chapter 3 Chromatography 12
  Section 1 Adsorption chromatography 12
  Section 2 Partition chromatography 12
  Section 3 Ion-exchange chromatography 14
  Section 4 Gel filtration 16
  Section 5 Affinity chromatography 18
  Chapter 4 Electrophoretic techniques 20
  Section 1 Basic principles 20
  Section 2 Several types of commonly used electrophoresis 23
  Chapter 5 Centrifugation techniques 29
  Section 1 Basic principle 29
  Section 2 General description of centrifugation 30
  Chapter 6 Genetic engineering and analytical technology of gene expression 34
  Section 1 Genetic engineering 34
  Section 2 Polymerase chain reaction 40
  Section 3 Nucleic acid hybridization 45
  Section 4 DNA sequencing technology 46
  Part 2 Experiments of the biochemistry and molecular biology
  Chapter 7 Analysis of purification and physicochemical properties of proteins 49
  Experiment 1 Colorimetric methods for protein determination 49
  Experiment 2 Isolation and purification of serum y-globulin 51
  Experiment 3 Cellulose acetate membrane electrophoresis of serum proteins 53
  Experiment 4 Determination of relative molecular mass of protein in SDS-PAGE 55
  Experiment 1 The specificity of enzyme and effect of temperature, pH on the enzyme Activity 58
  Experiment 2 Isolation of alkaline phosphatase and determination of its specific activity 60
  Experiment 3 Effects of substrates concentration of enzymatic activity: determination of Xm of alkaline phosphatase 64
  Experiment 4 Inhibition effect of phosphate on alkaline phosphatase 66
  Experiment 5 Analysis of lactic dehydrogenase isoenzyme 67
  Chapter 9 Substance metabolism 70
  Experiment 1 Extraction and identification of liver glycogen 70
  Experiment 2 Determination of alanine transaminase activity (Reitman-Frankels’method) 71
  Experiment 3 Enzyme catalyzed transamination of amino acids detected by paper chromatography 73
  Chapter 10 Basic experiment of molecular biology 76
  Experiment 1 Preparation of the genomic DNA in the eukaryotic cells 76
  Experiment 2 Determination of the concentration and purity of DNA by spectroscopy 77
  Experiment 3 Agarose gel electrophoresis of nucleic acids 77
  Experiment 4 Polymerase chain reaction (PCR) 79
  Experiment 5 Isolation and purification of the DNA fragments 80
  Experiment 6 DNA ligation^constructing recombinant plasmids 82
  Experiment 7 Preparation of E.coli competent cells 83
  Experiment 8 Transformation of Ca2+ competent cells 84
  Experiment 9 The screening of recombinant DNA 85
  Experiment 10 Minipreparation of plasmid DNA 86
  Experiment 11 Digestion of plasmid DNA with restriction endonucleases 89
  Experiment 12 Southern blot 90
  Experiment 13 Expression of exogenous gene in prokaryotic cells 93
  References 95
  Appendix 96
  Appendix Ⅰ The calculation table of ammonium sulfate saturation 96
  Appendix Ⅱ Common buffer solution preparation 96
  Appendix Ⅲ The relative molecular mass and pi of protein 100
  Appendix Ⅳ Common data of chromatography 101
  Appendix Ⅴ The conversion between centrifugation speed of centrifugation force 103