目录 前言 第一章生物化学常规技术训练 (1) 实验1蛋白质的沉淀和分离———离心技术训练 (1) Experiment 1Precipitation and Separation of Proteins—Operation of Centrifuge(4) 实验2硫酸铜的浓度测定———比色技术训练 (8) Experiment 2Determination of CuSO4 Concentration—Spectrophotometry Technique (13) 实验3血清蛋白质醋酸纤维薄膜电泳定量分析 (19) Experiment 3Quantitative Analysis of Serum Proteins by Cellulose Acetate Membrane Electrophoresis (29) 实验4层析技术训练 (43) Experiment 4Ion Exchange Chromatography (46) 第二章基础性实验 (50) 实验5蛋白质的分段盐析与透析 (50) Experiment 5Protein Salt Precipitation and Dialysis (52) 实验6双缩脲法测定血清总蛋白 (55) Experiment 6Determination of the Serum Total Proteins by Biuret Method (57) 实验7酪蛋白等电点测定 (60) Experiment 7Determination of the Isoelectric Point of Casein (62) 实验8唾液淀粉酶专一性以及温度等因素对酶活性的影响 (64) Experiment 8Specificity of Salivary Amylase and the Factors Affecting the Enzyme Activities (68) 实验9乳酸脱氢酶的递氢作用 (72) Experiment 9The Hydrogen Transferring Effect of Lactate Dehydrogenase (74) 实验10血浆胆固醇总量的测定(硫铁磷法) (76) Experiment 10Determination of Plasma Total Cholesterol with Sulphuriron-phosphorus Reagent (78) 实验11血清谷丙转氨酶(GPT / ALT)活性测定 (81) Experiment 11Determination of the Activity of Glutamate-Pyruvate Transaminase (GPT / ALT)in Serum (83) 实验12血浆二氧化碳结合力的测定———Van Slyke 酸碱滴定法 (86) Experiment 12Determination of CO2 Combining Power of Plasma—Van Slyke Acid-alkali Titration (88) 实验13酮体的生成和检测 (90) Experiment 13Detection of Ketogenesis in Liver (92) 实验14PCR 扩增髓鞘碱性蛋白基因 (94) Experiment 14Amplification of Myelin Basic Protein Gene by the Polymerase Chain Reaction(PCR) (96) 实验15DNA 琼脂糖凝胶电泳 (99) Experiment 15Agarose Gel Electrophoresis of DNA (101) 第三章综合性实验 (104) 实验16血清免疫球蛋白的分离、纯化和鉴定 (104) Experiment 16Separation,Purification and Identification of Serum Immunoglobulin (107) 实验17碱性磷酸酶的分离纯化及其Km 值测定 (110) Experiment 17Isolation of Alkaline Phosphatase and Determination of its Michaelis Constant (Km) (115) 实验18质粒DNA 的分离纯化与鉴定 (120) Experiment 18Isolation,Purification and Identification of Plasmid DNA (122) 实验19动物肝脏中DNA 的提取和检测 (124) Experiment 19Extraction and Detection of DNA from Liver of Swine (126) 实验20人肝癌细胞株HepG2 中内参GAPDH 的免疫印迹检测 (128) Experiment 20Expression of Reference GAPDH in Human Hepatoma Cell Lines HepG2 by Western Blot (132) 实验21肌糖原的酵解作用 (136) Experiment 21Glycolysis of Muscle Glycogen (139) 实验22水果中维生素C 的含量测定及多种因素对样品中维生素C 影响作用的 动态观察 (142) Experiment 22The Spoiling Effects of Heat Temperature,Alkali and Heavy Metal Ions on Vitamin C (146) 第四章设计性实验 (150) 实验23基因克隆技术-人髓鞘碱性蛋白基因(MBP)重组质粒的构建与鉴定 (150) Experiment23DNA Cloning-Construction and Identification of Recombinant Plasmid of Human Myelin Basic Gene(MBP) (154) 实验24人基因多态性分析 (158) Experiment 24Genetic Polymorphism Analysis of Human Gene (163) 参考文献 (168) 附录:实验室安全须知 (169) Appendix:Laboratory Safety (169）
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